11％井冈霉素•已唑醇悬浮剂在水稻上的残留动态研究(9000字)
摘 要：建立了井冈霉素在土壤、水稻植株、稻谷和糙米的残留分析方法：样品甲醇与水的混合提取剂(体积比为9∶1)，经C18 SPE小柱净化，用液相色谱（UV）检测，外表法定量。结果表明：添加水平在0.1 mg/Kg -5mg/Kg 范围之间，井冈霉素土壤中平均回收率为89.2%-89.7%，相对标准偏差4.5%-9.3%；当添加在水平在0.5 mg/Kg -5 mg/Kg范围之间在水稻植株中的平均回收率83.3%-90.1%，相对标准偏差3.0%-6.5%；当添加在水平在0.5mg/Kg-5mg/Kg范围之间在糙米中的平均回收率为85.1%-87.7%，相对标准偏差4.1%-7.5%，谷壳中的平均回收率为84.0%-86.2%，相对标准偏差4.1%-8.9%。井冈霉素的最小检出量(LOD)为0.1×10-9 g,在植株、糙米和谷壳的最低检测浓度(LOQ)为0.1 mg/kg。一年三地的残留试验结果表明, 井冈霉素在湖南省、广西区、福建省土壤和水稻植株中的半衰期分别为2.9天和1.7天、1.7天和1.6天和1.5天和1.6天。无论是高浓度区(750g/hm2)(有效成分)还是低浓度区( 375 g/hm2) (有效成分),水稻收获时,土壤和糙米样品中均未检出井冈霉素。
关键词：井冈霉素；残留分析；水稻
11% Jinggangmycin; Sc already thiazole alcohol on rice residue dynamic research
Abstract：Jingangmycin was established in soil and group of husked rice plants, rice and residual analysis methods: the mixture of methanol and water sample extraction agents (volume for 9:1) than by using C18 column purification, using SPE small liquid chromatography (UV) detection, appearance method quota. The results showed that to add level in 5mg/Kg range 0.1 mg/Kg - in the soil, interact between the average recovery of Jingangmycin 89.2%-89.7%, relative standard deviation 4.5% - 9.3 %per cent; When adding in horizontal in 0.5-5 range between the average recovery in rice plant 83.3%-90.1%, relative standard deviation 3.0-6.5 percent; When adding in horizontal in 0.5-5 range between the average recovery is in rice 85.1 - explaination 4.1%, relative standard deviation of the chaff 7.5%, the average recovery of 84.0% - 86.2%, relative standard deviation 4.1%t - 8.9%. Interact with minimum detected LOD (amount) 0.1 x 10-9 g, in plant, brown rice and chaff minimum detection concentration (LOQ) 0.1 mg/kg. A year in the three remaining test results show that in Hunan province, GuangXiOu Jingangmycin interact, Fujian province soil and rice plant respectively the half-life 2.9 d and
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1.7 d, 1.7 d and 1.6 d and 1.5 d and 1.6 d. Whether 750g/hm2 high concentration area (effective components) or (low concentration (375 g/hm2) (effective component), rice harvest in the sample, soil and brown rice was not detected in jingangmycin interact.
Key words：interact amphotericin, residues analysis, rice
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