mPEG修饰猪血红蛋白的制备与检测

mPEG修饰猪血红蛋白的制备与检测(12000字)
摘 要：脱离红细胞后的血红蛋白四聚体结构不稳定、氧亲和力强、半衰期短，还会引起一定的肾毒性。化学修饰作为一种新兴技术，能有效的改善上述不良特性。无基质猪血红蛋白（SFH）由新鲜猪血出发，经分离纯化制得。SC-mPEG大分子化pHb是延长其在循环系统中的停留时间、降低免疫原性、减少肾脏毒性的有效方法。幼犬的安全性实验表明产品无毒性，论证了其作为红细胞代用品的生物安全性。
关键词: 新鲜猪血；血红蛋白；化学修饰；聚乙二醇；红细胞代用品 

Preparation and detection of the pig hemoglobin modified by mPEG
Abstract：Hemoglobin,when it is departed from red blood cell,has many disadvantages such as structure instability,high oxygen affmity,short half-life and kidney toxicity.As a burgeoning technology,chemical modification of hemoglobin has been applied to improve these poor characteristics of the hemoglobin.Stroma-free porcine hemoglobin is separated and purified from fresh pig blood by centrifugation.Increasing the total molecular weight of hemoglobin theough SC-mPEG coupling has been proved to be a good choice for prolonging vascular retention time,and it can reduce the immunogenicity and the kidney toxicity . Animal experiments with dogs showed their biological safety.
Key words:fresh pig blood；hemoglobin；chemical modification；PEG；red blood cell substitute.