国内不同品系兔子多克隆抗体制备
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国内不同品系兔子多克隆抗体制备(毕业论文6500字)
摘 要:为提高制备多克隆抗体的效率和减少损失,通过对北方兔种和南方兔种的兔子进行针对性的免疫,根据观察兔子在免疫过程中死亡率的方法来确定何种品系的兔子能够大量产生优质血清,从实验结果得出南方兔种死亡率低于北方兔种,最后根据结果得出南方兔种更加适合进行多克隆抗体的制备。
关键词:多克隆抗体;免疫;死亡率;
DOMESTIC DIFFERENT STRAINS RABBIT POLYCLONAL ANTIBODY PREPARATION
Abstract:The company in order to improve the efficiency of polyclonal antibody preparation and reduce the loss, through to the domestic different versions of the rabbit Numbers by observing specific immune, the rabbit to determine what strains of death can erupt quality rabbit serum, according to the last nine weeks after that TuZhong more southern rabbit mortality for the preparation of polyclonal antibody, thus the reduction in need of enormous amounts of quality and serum for polyclonal antibody preparation time and loss.
Key words:Polyclonal antibody;immune; mortality;
目录
摘 要: 1
关键词: 1
1 前言 2
1.1多克隆抗体的概念 2
1.2免疫动物 3
1.3佐剂的应用 4
1.4抗体的鉴定 5
1.5采血 6
1.6 血清的分离 7
1.7抗血清的冻存 7
2 材料与方法 7
2.1 材料与试剂 7
2.2 免疫方法 7
2.3 取血 7
2.4 免疫 8
2.5 死亡率的测定 8
3 结果与分析 8
4 结论 9
参考文献 9
致谢 10 |