土壤中阿维菌素持久性研究

土壤中阿维菌素持久性研究(8500字)
摘  要：采用田间试验方法，研究了阿维菌素在柑橘园土壤中的残留消解动态，应用高效液相色谱法测定了阿维菌素园在壤中的残留量。研究了阿维菌素在土壤中的残留 动态。介绍了阿维菌素在土壤样品中的提取、净化以及液相色谱的分析测定方法。结果表明：阿维菌素标液浓度在0．0103-10．30 mg／L范围内，标液浓度与峰面积响应值呈良好的线性关系。阿维菌素在土壤中的平均添加回收率在80．0％-96．2％范围内，土壤中的最低检测浓度为 0．01 mg／kg。维菌素在土壤中的消解动态实验结果表明：阿维菌素在土壤中的消解符合一级化学反应动力学模式C=C0e-kt，长沙、贵州、杭州三地土壤的半 衰期分别为3.24d、4.53d、7.09d。该药属于易分解农药（T1/2＜30d），符合阿维菌素残留的检测要求。
关键词：高效液相色谱；阿维菌素；残留分析；土壤

Study of avermectin in soil persistence
Abstract: A field experiment was conducted to reveal the residue dynamics and residues of avermectin in citrus of soil．The residue content and degradation dynamics of avermectin in citrus and soil were studied by(HPLC) method．which  avermectin  residue in  soil dynamics. Introduced  in rice blast spirit of extraction and purification of soil and the HPLC analysis method。The residue content and degradation dynamics of avermectin in citrus and soil were studied by(HPLC) method．Avermectin showed good linear regression in the range of 0．0103-10．30 mg／L in sample solution．The soil avermectin the digestion dynamics experimental results show that: avermectin the digestion in citrus chemical reaction dynamics model with level C = C0e - kt, changsha, guizhou, hangzhou the half-life of three respectively soil 3.24 d、4.53 d、7.09 d. The half-life of IMI implied that it belonged to non-persistent pesticides(T1/2＜30d)。Thees tablished quantitative determ ination method was available with O．O1 mg／kg LOQ in soil．
Key words:：HPLC；avermectin；residue analysis；soil
 

目   录  
摘要 …………………………………………………………………………………1
    关键词…………………………………………………………………………………1
    1前言…………………………………………………………………………………2
    2试验材料与方法……………………………………………………………………3
2.1主要试验材料……………………………………………………………………3
2.3  主要试剂…………………………………………………………………3
2.3主要仪器与装置……………………………………………………………4
2.4  田间试验设计……………………………………………………………4
2.4.1柑橘园中土壤采集 ……………………………………………………………4
2.4.1柑橘园中土壤样品的制备 ……………………………………………………4
2.5 残留量测定方法…………………………………………………………………4
2.5.1  液相色谱条件选择…………………………………………………………4
2.5.2 标准曲线绘制…………………………………………………………………4
2.5.3  样品提取……………………………………………………………………5             
2.5.4 准确度和精密度试验…………………………………………………………5
3 实验结果分析与讨论………………………………………………………………5
3.1  检测方法………………………………………………………………………5
3.1.1  色谱条件选择………………………………………………………………5
3.1.2  线形范围和检测限…………………………………………………………5
3.2  准确度和精密度试验……………………………………………………6
3.3阿维菌素在土壤中的动态残留试验……………………………………………… 6
3.3.1  消解动态实验结果及分析…………………………………………………6
3.3.2  消解动态实验三地综合分析………………………………………………10
3.3.3 最终残留分析………………………………………………………………10
    4结束语………………………………………………………………………………11
    参考文献 ……………………………………………………………………………11
    致谢…………………………………………………………………………………13