光身稻基因精细定位之重组体的筛选

摘  要：光身稻是一种古老的分布广泛的重要的陆稻品种。目前关于光身稻的研究主要集中在育种方面，它被认为一种优质的稻种资源受到育种家的喜爱。另外也有少量的关于光身稻的生理生化和显微镜观察等的研究，而分子生物学方面的研究非常少而且进展不快，光身基因还没被克隆，也没有对该基因进行深入的研究。本论文是在光身稻初步定位的基础上利用双引物进行分子标记的方法，通过对一万株初步定位的群体进行筛选，找到了119株重组体，为接下来的精细定位提供了材料。
关键词：水稻；光身基因；重组体；筛选

The Screening for Recombinant of Glabrous Rice Gene on Fine-mapping
Abstract: Glabrous Rice is a kind of old distribution of a wide range of important Upland Rice. At present, about the research of Glabrous Rice mainly focused on breeding, as it is considered one of the highest quality rice resources attracted breeders. Also there are a few researches on its physiological and microscope observation etc, but the study of molecular biology very little and the dissertation is not much. Glabrous Gene hasn't been cloned, and about the research of the gene hasn't in-depth. This project is biased on preliminary positioning of Glabrous Rice, find 119 trees recombinant through the group of ten thousands trees of preliminary positioning screening by molecular marker with double primer, provided material for next fine-mapping.
Key words: rice; Glabrous Gene; Recombinant; screening

目    录     10000字
摘要……………………………………………………………………………………1
关键词…………………………………………………………………………………1
1  前言………………………………………………………………………………2
2  材料与方法………………………………………………………………………5
2.1  实验材料…………………………………………………………………5
2.1.1  供试的水稻材料……………………………………………………5
2.1.2  PCR引物……………………………………………………………5
2.1.3  主要仪器设备………………………………………………………5
2.1.4  主要试剂……………………………………………………………6
2.1.5  主要溶液及试剂配方………………………………………………6
2.2  实验方法…………………………………………………………………6
    2.2.1  目的材料的获取……………………………………………………6
    2.2.2  制备PCR模板DNA………………………………………………7
    2.2.3  PCR反应……………………………………………………………7
    2.2.4  聚丙烯酰胺凝胶电泳………………………………………………8
    2.2.5  银染显色……………………………………………………………9
3  结果与分析………………………………………………………………………9
3.1  读取数据…………………………………………………………………9
3.2  重组体确认………………………………………………………………11
    3.2.1  微量DNA提取……………………………………………………11
    3.2.2  PCR反应…………………………………………………………11
    3.2.3  聚丙烯酰胺凝胶电泳……………………………………………12
    3.2.4  银染显色…………………………………………………………12
    3.2.5  数据分析…………………………………………………………12
    3.2.6  移栽重组体………………………………………………………13
4  讨论………………………………………………………………………………13
5  结论………………………………………………………………………………15
参考文献……………………………………………………………………………16
致谢…………………………………………………………………………………18