拟南芥转座子插入突变体CS27715的筛选及形态学观察

摘  要：拟南芥是一种模式植物，在植物学研究中有重要意义。本试验是对拟南芥CS27715（CS25）基因突变体材料进行筛选, 通过育种发芽、提取DNA、PCR扩增和琼脂糖凝胶电泳等步骤筛选突变体，获得了CS25杂合子突变体。对CS25杂合子表型进行了初步形态学观察，结果显示：CS25杂合子突变体植株与野生型植株相比较，突变体植株矮小；叶片少，叶片表皮毛数量少，叶缘全缘；花柄短，花序集中；果荚长短径比值小；种子呈短椭圆型。
关键词：拟南芥；转座子CS25突变体；筛选

Screening of Transposable Elements Insertion Mutant（CS27715）in Arabidopsis and Morphology Observation 
Abstract：Arabidopsis is a pattern of plants and it has a great significance in botany research. In this study, we screened the heterozygous and homozygotes of CS27715 (CS25) heterozygous in Arabidopsis by PCR amplification. Then, with a particular morphology observation, the result showed that CS25 heterozygous mutant plants compared with wild-type plants, mutant plants stunted; leaves small, leaf table fur small, entire leaf edges; flower handles short, inflorescence concentrated; the pod is length ratio of small diameter; seeds are short elliptic.
Keywords: Arabidopsis; Transposon insertion mutation CS25; Screening

目    录    8000字
摘要………………………………………………………………………………………1
关键词………………………………………………………………………………………1
1前言……………………………………………………………………………………1
    1.1拟南芥简介………………………………………………………………………2
1.2转座子和转座子突变体的介绍………………………………………………2
        1.2.1 转座子介绍………………………………………………………………2
        1.2.2 转座子突变体介绍………………………………………………………3
2材料与方法…………………………………………………………………………4
    2.1材料……………………………………………………………………………4
    2.1.1试验材料…………………………………………………………………4
    2.1.2试验试剂…………………………………………………………………4
    2.1.3主要仪器…………………………………………………………………4
2.2方法………………………………………………………………………………4
        2.2.1材料处理…………………………………………………………………4
        2.2.2 DNA的抽提………………………………………………………………5
        2.2.3提取的基因组DNA电泳检测…………………………………………5
        2.2.4引物的设计与合成………………………………………………………5
        2.2.5 PCR扩增…………………………………………………………………6
        2.2.6 PCR产物的电泳检测……………………………………………………7
3结果与分析………………………………………………………………………………7
    3.1拟南芥CS25突变体（杂合子）和野生型植株形态特征………………7
    3.2 拟南芥CS25突变体（杂合子）和野生型生长状态区别………………11
    3.3 基因组DNA提取后的电泳检测结果………………………………………11
3.4 PCR产物电泳检测结果……………………………………………………12
4讨论…………………………………………………………………………………12
5结论……………………………………………………………………………13
参考文献 ………………………………………………………………………13
致谢……………………………………………………………………………14