木霉菌抑制姜瘟病的效果与拮抗机制研究

木霉菌抑制姜瘟病的效果与拮抗机制研究(含选题审批表,任务书,开题报告,中期检查表,毕业论文11000字)
摘 要：为研究木霉对姜瘟病的拮抗作用，本试验从湖南江永县的染病姜田中采集土壤分离出细菌6株，将分离出的菌株对新鲜健康的江永县生姜进行致病性的测定实验，筛选出致病菌BG菌株。通致病特征，过形态特征，革兰氏染色，以及鞭毛染色观察，初步鉴定此致病菌株BG为Ralstonia solanacearum。通过13号木霉菌株的室内对峙试验得证，木霉对致病菌株有较强的抑制作用。

关键词：姜瘟病；Ralstonia solanacearum；木霉；生物防治；拮抗作用

Study on Impact of Trichoderma spp. to Control Ginger Wilt Disease and Mechanism of its Antagonism 
Abstract: Soil samples were collected from the ginger cultivating district with the symptom of ginger wilt disease in Jiangyong county,Hunan province. 6 bacteria were isolated from these soil samples.Via experiment of bacteria's pathogenicity to ginger,  pathogen BG was screened out. The morphological characteristics observation in PDA, Gram stain solution, flagella staining of bacteria BG were conducted, and according to large numbers of references, we were able to preliminarily identify this bacteria BG were belong to Ralstonia solanacearum. Through the inhibitory activities that Trichoderma spp. to pathogen BG, the Trichoderma spp. has intense inhibitory effects on pathogen BG.
Keyword: ginger wilt disease；Ralstonia solanacearum；Trichoderma spp.；biological control；antagonism

目  录
摘要……………………………………………………………………………1
关键词…………………………………………………………………………1
1前言……………………………………………………………………………2
    1.1 致病菌的研究 ……………………………………………………………2
    1.2 致病机制的研究 …………………………………………………………3
        1.2.1致病因素………………………………………………………3
        1.2.2致病菌的侵染途径…………………………………………………4
    1.2.3致病机制……………………………………………………………5
    1.3木霉拮抗菌………………………………………………………………6
    1.4木霉拮抗机制………………………………………………………………6
2实验部分……………………………………………………………………7
    2.1材料与方法 ………………………………………………………………7
        2.1.1试验样品……………………………………………………………7
        2.1.2 试剂…………………………………………………………………7
        2.1.3 仪器…………………………………………………………………7
    2.1.4 培养基………………………………………………………………7
    2.2 方法……………………………………………………………………7
    2.2.1 土壤样品的采集与分离……………………………………………7
        2.2.2 从土壤样品中分离疑似致病菌……………………………………7
    2.2.3对分离菌株进行病原致病性测定………………………………8
    2.2.4 致病菌的初步鉴定…………………………………………………8
    2.2.5 木霉对姜瘟病致病菌的拮抗作用…………………………………9
    2.2.4 木霉拮抗作用机制的观察…………………………………………9
3 结果与分析…………………………………………………………………………9
    3.1 姜瘟病致病菌致病性的测定结果…………………………………………9
3.2致病菌的鉴定结果………………………………………………10
3.3 木霉对姜瘟病致病菌的拮抗作用………………………………………11
3.4 木霉拮抗作用机制观察结果……………………………………………13
4 讨论 ………………………………………………………………………13
参考文献 ………………………………………………………………………14
致谢……………………………………………………………………………17