木霉菌生长速度测定及几丁质酶活力测定

木霉菌生长速度测定及几丁质酶活力测定(含选题审批表,任务书,开题报告,中期检查表,毕业论文8600字)
摘  要：本实验通过在不同的时间段用“十字交叉法”对木霉菌在培养皿上生长半径的测定，对木霉菌自身的生长状况进行研究；以及对不同木霉菌株在相同发酵条件下（28℃,180r／min）产几丁质酶的酶活力大小进行测定，最终确定木霉菌的最适发酵条件。这些结果可以作为今后进一步研究木霉特性的依据，有助于更好的了解木霉对各种病原菌株的拮抗作用机制，也为以后开发生物产品提供了数据参考。
关键词：木霉；几丁质酶；对峙实验.

The Assay Growth Rate of Trichoderma and Chitinase Enzyme Activity
Abstract: The growth of Trichoderma under different period in culture plate ware determined .And the enzyme activity of Chitinase under some fermentation condition was studied too. To various sorts of Trichoderma .Then the optimum fermentations conditions was confirmed .Result shows that the dates can be used as buttress for the subsequent study on Trichoderma characteristics. And research of biological products development, also the dates were beneficial to understand the way the antagonism of Trichoderma to various pathogens.
Key words: Trichoderma；Environmental conditions；Pepper Sclerotium rolfsii disease

目  录
摘  要    1
关键词.    1
1 前言    1
2 材料与方法    6
2.1 材料    6
2.1.1 实验材料    6
2.1.2 主要仪器与试剂    6
2.2 方法    7
2.2.1  11株不同木霉生长速度的测定    7
2.2.2  几丁质酶活力的测定    7
3 结果与分析    9
3.1 木霉生长情况    9
3.2  N-乙酰氨基葡萄糖量（NAG）标准曲线    10
3.3  不同的木霉菌株产几丁质酶活力的测定    10
4 讨论    12
参考文献    12
致  谢    14