高油辣椒制品中辣椒素类物质的测定

高油辣椒制品中辣椒素类物质的测定(6900字)

摘  要：本文初步建立了HPLC(High performance liquid chromatographic method高效液相色谱法)测定高油辣椒制品中辣椒制品中两种主要辣椒素类物质的方法。样品以甲醇+正己烷进行预处理，采用反相高效液相色谱分离,紫外检测器;根据色谱峰的保留时间定性,以外标峰面积法定量辣椒素(C)、二氢辣椒素(DC)。采用色谱柱,ZORBAX SB-C18;流动相,V(甲醇)∶V(水)=65：35；UV检测波长,280 nm;辣椒素和二氢辣椒素RSD分别为5.11%,5.08%(n=5);回收率分别为89.4%、90.0%。辣椒素和二氢辣椒素在20－200ug/ml范围内线性良好。方法满足辣味水平评价要求。
关键词：辣椒；高油含量；HPLC；测定

DETERMINATION OF CAPSAICIN IN THE HIGH OIL PEPPER PRODUCTS

Abstract：A method was preliminary developed for the determination of capsaicinoids in highly oil-containing pepper productsby high performance liquid chromatography. The sample was extractedwith Methanol and Hexane. The analysis wasperformed on a C18 column (250×4.6 mm i.d.5μm) with 65∶35 Methanol-water as the mobile phase at a flow rate of 1.2 mL/min and detected at 280 nm. The determination of capsaicin(C),dihydrocapsaicin(DC) were processed by peak area andexternal standard method. The precisions of C and DC were good with the RSD of 5.11% and 5.08% espectively (n=5).The recoveries for C and DC are 89.4% and 90%respectively. The two ompounds demonstrated good linear relationship in the range of 20-200 ug/ml.

Key words：Pepper, High oil content, HPLC, Determine
 

目    录


摘  要    1
关键词    1
1  前言    2
2  材料与方法    3
2.1  材料    3
2.2  主要试剂    3
2.3  主要仪器设备    3
2.4  试验方法    3
2.4.1  样品提取溶剂选择性实验    3
2.4.2  正己烷最佳加入量的确定    4
2.4.3  高效液相色谱法条件选择试验    4
2.4.4  样品预处理    4
2.4.5  对照处理     4
2.5  标准溶液配制及绘制标准曲线.    5
2.6  结果计算    6
2.6.1  辣椒素物质总量的计算    6
2.6.2  二氢辣椒素物质总量的计算    6
3  结果与分析    6
3.1辣椒素提取剂的确定    6
3.2  正己烷加入量的确定    6
3.3  高效液相色谱法条件确定    7
3.3.1  流动相的确定    7
3.3.2  流速的确定    8
3.3.3  UV检测波波长的选择    8
3.3.4色谱条件    8
3.4  线性关系    8
3.5 回收率试验    9
3.6  精密度试验     9
3.7  样品测定结果    10
4  结论    10
参考文献    10
致    谢    12